Miniturized chip for free-flow-electrophoresis
For the detection and quantitative analysis of liquid mixture ingredients, for monitoring chemical and biological production and purification processes as well as for analytical procedures in medical diagnostics, the department Photonic Sensor Technology has developed a continuous online analysis and monitoring process. The current focus lies in the monitoring of protein production for pharmaceutical applications.
The method is based on the coupling of free-flow electrophoresis (µFFE), in which the components of a complex liquid mixture are separated, and an optical detection system, such as fluorescence or surface-enhanced Raman scattering (SERS). The detection system is highly sensitive and is used for the identification of the different molecular species being separated and enriched by µFFE.
The miniaturization of the FFE system has some advantages. In particular, μFFE-systems require only a few nanoliters to microliters of a sample, which makes the application in clinical analysis or protein production, where only small amounts of samples are available, particularly interesting. Another important advantage of miniaturization is the avoidance of heating due to the otherwise occurring high electrical currents. The chip is produced and sold by microfluidic ChipShop.
Walowski, B., Hüttner, W., Wackerbarth, Generation of a miniaturized free-flow electrophoresis chip based on a multi-lamination technique—isoelectric focusing of proteins and a single-stranded DNA fragment, Analytical and Bioanalytical Chemistry, 401, 2465-2471, 2011.
Hüttner, W., Christou, K., Göhmann, A., Beushausen, V., Wackerbarth, H., Implementation of substrates for surface-enhanced Raman spectroscopy for continuous analysis in an optofluidic device, Microfluid Nanofluid, 12 (1-4), 521-527, 2012.
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Laser-Laboratorium Göttingen e.V. (LLG)
Head of Department
Dr. Hainer Wackerbarth
"Photonic Sensor Technologies"