2018

Publications

  • R. Kowarsch, C. Geisler, A. Egner, C. Rembe:
    Superresolution reflection microscopy via absorbance modulation: A theoretical study,
    Opt. Ex. 26 (5) (2018)
  • B. Roubinet, M. Bischoff, S. Nizamov, S. Yan, C. Geisler, S. Stoldt, G. Y. Mitronova, V. N. Belov,
    M. L. Bossi, S. W. Hell:
    Photoactivatable Rhodamine Spiroamides and Diazoketones Decorated with “Universal Hydrophilizer” or Hydroxyl Groups, J. Org. Chem. 83, 12 (2018)
     
  • E. Neubert, D. Meyer, F. Rocca, G. Günay, J. Grandke, A. Kwaczala-Tessmann, S. Senger-Sander, C. Geisler, A. Egner, M. Schön, L. Erpenbeck, S. Kruss:
    Chromatin swelling drives neutrophil extracellular trap release, Nature Communications 9,1:3767 (2018)

2017

Conference constributions

  • R. Siegmund, C. Geisler, A. Egner:
    IsoSTED microscopy in living cells, SFB 755 Winterschool 2017,
    Hirschegg Kleinwalsertal, Österreich, (02.2017)
  • J.-R. Krüger, J. Keller-Findeisen, C. Geisler, A. Egner:
    Tomographic STED microsopy, CNMPB-Retreat,
    Bad Sooden-Allendorf (02.2017)
  • F. Rocca, N. Mazkereth, J. Krüger, C. Geisler, A. Egner, Z. Fishelson:
    Two-Color STED microscope for the analysis of stress-induced interaction
    between mitochondrial chaperone mortalin and the plasma membrane attack complex,
    Focus on Microscopy 2017, Bordeaux, France (04.2017)
  • J. Krüger, J. Keller-Findeisen, B. Vincon, C. Geisler, A. Egner:
    Tomographic STED microscopy, Focus on Microscopy 2017,
    Bordeaux, France (04.2017)
  • H. Mittelstädt, C. Geisler, A. Egner:
    Single marker switching microscope with isotropic resolution over large axial range,
    SPIE Optics + Photonics, Nanoscience + Engineering, Nanoimaging and Nanospectroscopy V,
    San Diego, CA, USA (08.2017)
  • J.-R. Krüger, J. Keller-Findeisen, C. Geisler, A. Egner:
    Tomographic STED microscopy,
    SPIE Optics + Photonics, Nanoscience + Engineering, Nanoimaging and Nanospectroscopy V,
    San Diego, CA, USA (08.2017)

2016

Publications

  • A. Gucek, J. Jorgacevski, P. Singh, C. Geisler, M. Lisjak, N. Vardjan, M. Kreft, A. Egner, R. Zorec:
    "Dominant negative SNARE peptides stabilize the fusion pore in a narrow, release-unproductive state", Cell. Mol. Life Sci. (2016).
  • S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, S. Jakobs:
    „Comment on "extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics". Science 352 (6285), 527-527, (2016)
  • N. Mazkereth, F. Rocca, J. Schubert, C. Geisler, Y. Hillman, A. Egner, Z. Fishelson:
    „Complement triggers relocation of Mortalin/GRP75 frommitochondria to the plasma membrane”, Immunobiology, (2016)

Conference constributions

  • H. Grefe, C. Geisler, A. Egner:
    Novel 3D single marker switching microscope with isotropic resolution over large axial range, SPIE Photonics West 2016, Single Molecule Spectroscopy and Superresolution Imaging IX, San Francisco, USA (02.2016)
  • A. Egner:
    New developments in optical nanoscopy, International Symposium 2016 "Biological Dynamics from Microscopic to Mesoscopic Scales", Grimma (02.2016)
  • R. Siegmund, C. Geisler, A. Egner:
    isoSTED microscopy in living cells, International Symposium 2016 "Biological Dynamics from Microscopic to Mesoscopic Scales", Grimma (02.2016)
  • H. Mittelstädt, C. Geisler, A. Egner:
    Single Marker Switching Microscope with Isotropic Resolution over Large Axial Range, Focus on Microscopy 2016, Taipei, Taiwan (03.2016)
  • J. Schubert, J. Keller-Findeisen, C. Geisler, B. Vincon, A. Egner:
    Low-intensity STED microscope with increased image brightness and uncompromised resolution, 80. Jahrestagung der DPG und DPG-Frühjahrstagung, Regensburg (03.2016)
  • A. Egner:
    Super-resolved fluorescence microscopy: Principle and applications of STED microscopy, BioImaging Symposium 2016, München (04.2016)
  • D. Köhne, C. Geisler, P. Simon, A. Egner:
    Principles and applications of optical switching assisted imaging and structuring schemes, International Conference on Physics 2016, New Orleans, USA (06.2016)
  • A. Egner, J. Schubert, C. Geisler, B. Vincon:
    Low-intensity STED microscope with increased image brightness and uncompromised resolution, SPIE Nanoscience + Engineering, Nanoimaging and Nanospectroscopy IV, San Diego, USA (08.2016)
  • A. Egner:
    Super-resolved fluorescence microscopy: Principle and applications of STED microscopy, 623th Wilhelm and Else Heraeus-Seminar on "Cellular Dynamics", Bad Honnef (09.2016)
  • R. Siegmund, F. Rocca, C. Geisler, A. Egner:
    isoSTED microscopy in living cells, International Symposium 2016 "Biological Dynamics from Microscopic to Mesoscopic Scales", Grimma (02.2016)
  • A. Egner, A. Munk:
    Statistical reconstruction methods for time varying nanoscale imaging problems, International Symposium 2016 "Biological Dynamics from Microscopic to Mesoscopic Scales", Grimma (02.2016)
  • B. Vinçon, J.-R. Krüger, C. Geisler, A. Egner:
    Ultrafast switchable depletion patterns for STED microscopy generated by conical diffraction, 623th Wilhelm and Else Heraeus-Seminar on "Cellular Dynamics", Bad Honnef (09.2016)
  • F. Rocca, N. Mazkereth, J.-R. Krüger, C. Geisler, A. Egner, Z. Fishelson:Complement triggers relocation of Mortalin/GRP75 from mitochondria to the plasma membrane, 623th Wilhelm and Else Heraeus-Seminar on "Cellular Dynamics", Bad Honnef (09.2016)

2015

Publications

  • A. Hartmann, S. Huckemann, J. Dannemann, O. Laitenberger, C. Geisler, A. Egner, A. Munk:
    Drift Estimation in sparse sequential dynamic imaging: with application to nanoscale fluorescence microscopy, J. R. Stat. Soc. B, doi: 10.1111/rssb.12128 (2015)
  • T. Aspelmeier, A. Egner, A. Munk:
    Modern Statistical Challenges in High-Resolution Fluorescence Microscopy, Annu. Rev. Stat. Appl. 2, 163–202 (2015)

Conference contributions

  • R. Siegmund, C. Geisler, F. Rocca, C. A. Wurm, S. Jakobs, A. Egner:
    Three Dimensional IsoSTED Microscopy In Living Cells, Focus on Microscopy 2015, Göttingen (03.2015)
  • O. Laitenberger, A. Hartmann, C. Geisler, A. Munk, A. Egner:
    Statistical Drift Estimation And Correction Methods For Single Marker Switching Based Microscopy, Focus on Microscopy 2015, Göttingen (03.2015)
  • H. Grefe, C. Geisler, A. Egner:
    Novel 3D Single Marker Switching Microscope With Isotropic Resolution Over Large Axial Range, Focus on Microscopy 2015, Göttingen (03.2015)
  • A. Egner, C. Geisler, H. Grefe, J. Schubert:
    Developments in fluorescence nanoscopy, Optics + Photonics, Nanoimaging and Nanospectroscopy III, San Diego (08.2015)
  • R. Siegmund, C. Geisler, A. Egner:
    isoSTED microscopy in living cells, Optics + Photonics, Nanoimaging and Nanospectroscopy III, San Diego (08.2015)
  • A. Egner:
    Super-Resolved Fluorescence Microscopy: Principle and Applications of STED Microscopy, IMB seminar, Tromsø (10.2015)
  • A. Egner:
    Angewandte Optik – abbilden, analysieren und strukturieren mit Licht, Bier & Brezeln, Göttingen (11.2015)
  • J.-R. Schubert, C. Geisler, A. Egner:
    Low-Intensity STED Microscope With Increased Image Brightness And Uncompromised Resolution, Focus on Microscopy 2015, Göttingen (03.2015)

2014

Publications

  • A. Hartmann, S. Huckemann, J. Dannemann, O. Laitenberger, C. Geisler, A. Egner, A. Munk:
    Drift Estimation in sparse sequential dynamic imaging: with application to nanoscale fluorescence microscopy arxiv.org, 1403.1389, (2014)
     
  • V. N. Belov, G. Y. Mitronova, M. L. Bossi, V. P. Boyarskiy, E. Hebisch, C. Geisler, K. Kolmakov, C. A. Wurm, K. I. Willig, S. W. Hell:
    Masked Rhodamine Dyes of Five Principal Colors Revealed by Photolysis of a 2-Diazo-1-Indanone Caging Group: Synthesis, Photophysics, and Light Microscopy Applications, Chem. Eur. J. 20, 13162-13173 (2014)

Conference contributions

  • A. Egner:
    Overcoming the resolution barrier by STED microscopy Symposium on Advanced Microscopy Techniques in Life Sciences, Homburg (04.2014)
  • A. Egner:
    Three‐dimensional optical nanoscopy with opposing lenses SPIE Optics and Photonics, San Diego (08.2014)
  • A. Egner:
    Three‐dimensional optical nanoscopy with opposing lenses 18th International Microscopy Congress, Prague (09.2014)
  • A. Egner:
    Optical Nanoscopy meets Statistiks: Decoding the Building Blocks of Life Eröffnungssymposium des Felix‐Bernstein Institut für Mathematische Statistik in den Biowissenschaften, Göttingen, (10.2014)

2013

Conference constributions

  • A. Egner:
    "Three-dimensional optical nanoscopy with opposing lenses"
    Symposium on „Super Resolution Imaging“, Göttingen (2013)
     
  • C. Geisler, R. Schmidt, D. Aquino, S. W. Hell, A. Egner:
    "Developments in Fluorescence Nanoscopy", SPIE Optics + Photonics, San Diego (2013)
     
  • C. Geisler, T. Hotz, A. Schönle, S. W. Hell, A. Munk, A. Egner:
    "Drift estimation for single marker switching based imaging schemes"
    SPIE Optics + Photonics, San Diego (2013)
     
  • A. Egner:
    "Superresolution Microscopy"
    Fourth Annual Conference "Superresolution Microscopy", Ljubljana (2013)
     
  • C. Geisler, J. Jorgačevski, M. Kreft, R. Zorec, A. Egner:
    "Two-color STED microscopy"
    Fourth Annual Conference "Superresolution Microscopy", Ljubljana (2013)
     
  • A. Egner:
    "Overcoming the resolution barrier by STED microscopy"
    Visualizing the invisible in cell biology, Symposium on super-resolution microscopy, Prague (2013)

2012

Publications

  • C. Geisler, T. Hotz, A. Schönle, S.W. Hell, A. Munk, A. Egner:
    "Drift estimation for single marker switching based imaging schemes"  Opt. Express 20, 7274 (2012)
     
  • C. Geisler, T. Hotz, A. Schönle, S.W. Hell, A. Munk, A. Egner:
    "Drift estimation for single marker switching based imaging schemes"
    Opt. Express 20, 7274 (2012)
     
  • F. Opazo, M. Levy, M. Byrom, C. Schäfer, C. Geisler, T. W. Groemer, A. D. Ellington, S. O. Rizzoli:
    "Aptamers as potential tools for superresolution microscopy"
    Nat. Meth. 9, 938 (2012)
     
  • A. C. Meyer, D. Khimich, A. Egner, T. Moser:
    "Super-resolution optical microscopy of the organ of Corti.
    Investigations on the fine structure of the inner hair cell afferent synapse by the 4Pi and STED techniques."
    HNO 60, 707 (2012)
     
  • G. Whelan, E. Kreidl, G. Wutz, A. Egner, J. M. Peters, G. Eichele:
    "Cohesin acetyltransferase Esco2 is a cell viability factor and is required for cohesion in pericentric heterochromatin."
    EMBO J. 31, 71 (2012)

2011

Publications

  • D. Aquino, A. Schönle, C. Geisler, C. v. Middendorff, C. A. Wurm, Y. Okamura, T. Lang, S. W. Hell, A. Egner:
    "Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores"
     Nature Methods, 8 (4), 353 - 359, (2011)
     
  • Y. Okamura, R. Schmidt, I. Raschke, M. Hintze, S. Takeoka, A. Egner, T. Lang:
    "A Few Immobilized Thrombins Are Sufficient for Platelet Spreading"
     Biophysical Journal, Volume 100, Issue 8, (2011)
     
  • Y. Hua, R. Sinha, C. Thiel, R. Schmidt, J. Huve, H. Martens, S. W. Hell, A. Egner, J. Klingauf:
    "A readily retrievable pool of synaptic vesicles", Nature Neuroscience  doi:10.1038/nn.2838, (2011)
     
  • C. A. Wurm, D. Neumann, M. A. Lauterbach, B. Harke, A. Egner, S. W. Hell, S. Jakobs:
    "Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient"
    PNAS, doi:10.1073/pnas.1107553108, (2011)
     
  • K. Kumar, L. Isa, A. Egner, R. Schmidt, M. Textor, E. Reimhult:
    "Formation of Nanopore-Spanning Lipid Bilayers through Liposome Fusion!"
    Langmuir, 27, 10920-10928, (2011)
     
  • C. K. Ullal, S. Primpke, R. Schmidt, U. Böhm, A. Egner, P. Vana, S. W. Hell:
    "Flexible Microdomain Specific Staining of Block Copolymers for 3D Optical Nanoscopy"
    Macromolecules, 44, 7508-7510, (2011)

2010

Publications

  • G. Vicidomini, R. Schmidt, A. Egner, S. W. Hell, A. Schönle:
    "Automatic deconvolution in 4Pi-microscopy with variable phase"
     Opt. Exp. 18 (8), 10154 - 10167, (2010)
     
  • C. A. Wurm, D. Neumann, R. Schmidt, A. Egner and S. Jakobs:
    "Sample Preparation for STED Microscopy"
    In: D.B. Papkovsky (ed.), Live Cell Imaging, Methods in Molecular Biology 591, DOI 10.1007/978-1-60761-404-3 11, © Humana Press, a part of Springer Science+Business Media,
    LLC (2010)
     
  • T. Frank, M. A. Rutherford, N. Strenzke, A. Neef, T. Pangrsic, D. Khimich, A. Fejtova, E. D. Gundelfinger, M. C. Liberman, B. Harke, K. E. Bryan, A. Lee, A. Egner, D. Riedel, T. Moser:
    "Bassoon and the Synaptic Ribbon Organize Ca2+ Channels and Vesicles to Add Release Sites and Promote Refilling"
    Neuron, 68, 724-738, (2010)

2009

Publications

  • S. W. Hell,  R. Schmidt, A. Egner:
    "Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses" (Nature Photonics 3, 381 - 387), (2009)
     
  • A. C. Meyer, T. Frank, D. Khimich, G. Hoch, D. Riedel, N. M. Chapochnikov, Y. M. Yarin, B. Harke, S. W. Hell,
    A. Egner, T. Moser:
    "Tuning of synapse number, structure and function in the cochlea", Nature Neuroscience 12 (4), 444-453, (2009)
     
  • R. Schmidt, C. A. Wurm, A. Punge, A. Egner, S. Jakobs, S. W. Hell:
    "Mitochondrial Cristae Revealed with Focused Light", Nano Lett. 9 (6), 2508-2510, (2009)
     
  • C. K. Ullal, R. Schmidt, S. W. Hell, A. Egner:
    "Block Copolymer Nanostructures Mapped by Far-Field Optics", Nano Lett. 9 (6), 2497-2500 (2009)
     
  • G. Perinetti, T. Müller, A. Spaar, R. Polishchuk, A. Luini, A. Egner:
    "Correlation of 4Pi and Electron Microscopy to Study Transport Through Single Golgi Stacks in Living Cells with Super Resolution", Traffic 10, 379-391, (2009)

2008

  • R. Schmidt,  C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, S. W. Hell:
    "Spherical nanosized focal spot unravels the interior of cells"
    Nature Meth. 5 (6), 539 - 544 (2008)
     
  • M. Andresen, A. C. Stiel, J. Fölling, D. Wenzel, A. Schönle, A. Egner, C. Eggeling, S. W. Hell, S. Jakobs:
    "Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy"
    Nature Biotech. 26 (9), 1035-1040 (2008)
     
  • A. C. Stiel, M. Andresen, H. Bock, M. Hilbert, J. Schilde, A. Schönle, C. Eggeling, A. Egner, S. W. Hell, S. Jakobs:
    "Generation of Monomeric Reversibly Switchable Red Fluorescent Proteins for Far-Field Fluorescence Nanoscopy"
    Biophys. J. 95, 2989-2997 (2008)
     
  • M. Bossi, J. Fölling, V. N. Belov, V. P. Boyarskiy, R. Medda, A. Egner, C. Eggeling, A. Schönle, S. W. Hell:
    "Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species"
    Nano Letters 8 (8), 2463 - 2468 (2008)
     
  • C. v. Middendorff, A. Egner, C. Geisler, S. W. Hell, A. Schönle:
    "Isotropic 3D Nanoscopy based on single emitter switching"
     Opt. Expr. 16 (25), 20774-20788 (2008)
     
  • I. Testa, A. Schönle, C. v. Middendorff, C. Geisler, R. Medda, C. A. Wurm, A. C. Stiel, S. Jakobs, M. Bossi, C. Eggeling, S. W. Hell, A. Egner:
    "Nanoscale separation of molecular species based on their rotational mobility"
    Opt. Expr. 16 (25), 21093 - 21104 (2008)
     
  • J. Fölling, V. Belov, D. Riedel, A. Schönle, A. Egner, C. Eggeling, M. Bossi, S. W. Hell:
    "Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers"
    ChemPhysChem 9, 321 - 326 (2008)

2007

  • A. Egner,  C. Geisler, C. v. Middendorff, H. Bock, D. Wenzel, R. Medda, M. Andresen, A. C. Stiel, S. Jakobs, C. Eggeling, A. Schönle, S. W. Hell:
    "Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters", Biophys. J. 93, 3285 - 3290 (2007)
     
  • J. Fölling,  V. Belov, R. Kunetsky, R. Medda, A. Schönle, A. Egner, C. Eggeling, M. Bossi, S. W. Hell:
    "Photochromic Rhodamines provide Nanoscopy with Optical Sectioning"
    Angew. Chem. Int. Ed. 46, 6266 - 6270 (2007)
     
  • H. Bock, C. Geisler, C. Wurm, S. Jakobs, A. Schönle, A. Egner, S.W. Hell, C. Eggeling:
    "Two-color far-field fluorescence nanoscopy based on photoswitching emitters", Appl. Phys. B 88, 161-165 (2007)
     
  • C. Geisler,  A. Schönle, C. v. Middendorf, H. Bock, C. Eggeling, A. Egner, S.W. Hell:
    "Resolution of Lambda /10 in fluorescence microscopy using fast single molecule photo-switching", Appl. Phys. A 88, 223-226 (2007)
     
  • C. Eggeling, M. Hilbert, H. Bock, C. Ringemann, M. Hofmann, A. C. Stiel, M. Andresen, S. Jakobs, A. Egner, A. Schönle, S. W. Hell:
    "Reversible Photoswitching Enables Single-Molecule Fluorescence Fluctuation Spectroscopy at High Molecular Concentration"
    Micr. Res. Techn. 70, 1003 - 1009 (2007)

2006

  • A. Egner,  S. W. Hell:
    "Aberrations in Confocal and Multi-Photon Fluorescence Microscopy Induced by Refractive Index Mismatch"
    In: Handbook of Biological Confocal Microscopy, pp. 404-413, Ed. J. Pawley, Springer, New York (2006)
     
  • J. Bewersdorf, A. Egner, S. W. Hell:
    "Multifocal Multi-Photon Microscopy"
    In: Handbook of Biological Confocal Microscopy, pp. 550-560, Ed. J. Pawley, Springer, New York (2006)
     
  • J. Bewersdorf, A. Egner, S. W. Hell:
    "4Pi Microscopy"
    In: Handbook of Biological Confocal Microscopy, pp. 561-570, Ed. J. Pawley, Springer, New York (2006)

1998 - 2005

  • A. Egner,  S. W. Hell:
    "Fluorescence microscopy with super-resolved optical sections"
    Trends Cell Biol. 15 (4): 207 - 215 (2005)
     
  • A. Egner, S. Verrier, A. Goroshkov, H.-D. Söling and S. W. Hell:
    "4Pi-microscopy of the Golgi apparatus in live mammalian cells"
    J. Struct. Biol. 147(1): 70-76 (2004)
     
  • S. Jakobs, N. Martini, A. C. Schauss, A. Egner, B. Westermann, S. W. Hell:
    "Spatial and temporal dynamics of budding yeast mitochondria lacking division component Fis1p"
    J. Cell Sci. 116: 2005-2014 (2003)
     
  • A. Egner, S. Jakobs and S. W. Hell:
    "Fast 100-nm resolution 3D-microscope reveals structural plasticity of mitochondria in live yeast"
    Proc. Natl. Acad. Sci. USA 99: 3370-3375 (2002)
     
  • A. Egner, V. Andresen, S. W. Hell:
    "Comparison of the axial resolution of practical Nipkow-disk confocal fluorescence microscopy with that of multifocal multiphoton microscopy: theory and experiment"
    J. Microsc. 206: 24-32 (2003)
     
  • V. Andresen, A. Egner, S. W. Hell:
    "Time-multiplexed multifocal multiphoton microscope"
    Opt. Lett. 26(2): 75-77 (2003)
     
  • A. Egner and S. W. Hell:
    "Time multiplexing and parallelization in multifocal multiphoton microscopy"
    J. Opt. Soc. Am. A 17(7): 1192-1201 (2003)
     
  • T. Klar, S. Jakobs, M. Dyba, A. Egner, S. W. Hell:
    "Fluorescence microscopy with diffraction resolution limit broken by stimulated emission"
    PNAS 97(15): 8206-8210 (2000)
     
  • A. Egner, S. W. Hell:
    "Equivalence of the Huygens-Fresnel and Debye approach for the calculation of high aperture point-spread-functions in the presence of refractive index mismatch"
    J. Microsc. 193(3): 244-249 (1999)
     
  • A. Egner, M. Schrader, S. W. Hell:
    "Refractive index mismatch induced intensity and phase variations in fluorescence confocal, multiphoton and 4Pi-microscopy"
    Opt. Commun. 153: 211-217 (1998)

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Laser-Laboratorium Göttingen e.V. (LLG)

Contact person:

Head of department
Prof. Dr. Alexander Egner
"Optical Nanoscopy"

Tel.: +49(0)551/5035-35
FAX: +49(0)551/5035-99
alexander.egner(at)llg-ev.de