Publications

• R. Kowarsch, C. Geisler, A. Egner, C. Rembe:
  Superresolution reflection microscopy via absorbance modulation: A theoretical study,
  Opt. Ex. 26 (5) (2018)

• B. Roubinet, M. Bischoff, S. Nizamov, S. Yan, C. Geisler, S. Stoldt, G. Y. Mitronova, V. N. Belov,
  M. L. Bossi, S. W. Hell:
  Photoactivatable Rhodamine Spiroamides and Diazoketones Decorated with “Universal Hydrophilizer” or Hydroxyl Groups, J. Org. Chem. 83, 12 (2018)
   
• E. Neubert, D. Meyer, F. Rocca, G. Günay, J. Grandke, A. Kwaczala-Tessmann, S. Senger-Sander, C. Geisler, A. Egner, M. Schön, L. Erpenbeck, S. Kruss:
  Chromatin swelling drives neutrophil extracellular trap release, Nature Communications 9,1:3767 (2018)

Conference constributions

• R. Siegmund, C. Geisler, A. Egner:
  IsoSTED microscopy in living cells, SFB 755 Winterschool 2017,
  Hirschegg Kleinwalsertal, Österreich, (02.2017)

• J.-R. Krüger, J. Keller-Findeisen, C. Geisler, A. Egner:
  Tomographic STED microsopy, CNMPB-Retreat,
  Bad Sooden-Allendorf (02.2017)

• F. Rocca, N. Mazkereth, J. Krüger, C. Geisler, A. Egner, Z. Fishelson:
  Two-Color STED microscope for the analysis of stress-induced interaction
  between mitochondrial chaperone mortalin and the plasma membrane attack complex,
  Focus on Microscopy 2017, Bordeaux, France (04.2017)

• J. Krüger, J. Keller-Findeisen, B. Vincon, C. Geisler, A. Egner:
  Tomographic STED microscopy, Focus on Microscopy 2017,
  Bordeaux, France (04.2017)

• H. Mittelstädt, C. Geisler, A. Egner:
  Single marker switching microscope with isotropic resolution over large axial range,
  SPIE Optics + Photonics, Nanoscience + Engineering, Nanoimaging and Nanospectroscopy V,
  San Diego, CA, USA (08.2017)

• J.-R. Krüger, J. Keller-Findeisen, C. Geisler, A. Egner:
  Tomographic STED microscopy,
  SPIE Optics + Photonics, Nanoscience + Engineering, Nanoimaging and Nanospectroscopy V,
  San Diego, CA, USA (08.2017)

Publications

• A. Gucek, J. Jorgacevski, P. Singh, C. Geisler, M. Lisjak, N. Vardjan, M. Kreft, A. Egner, R. Zorec:
  "Dominant negative SNARE peptides stabilize the fusion pore in a narrow, release-unproductive state", Cell. Mol. Life Sci. (2016).

• S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, S. Jakobs:
  „Comment on "extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics". Science 352 (6285), 527-527, (2016)

• N. Mazkereth, F. Rocca, J. Schubert, C. Geisler, Y. Hillman, A. Egner, Z. Fishelson:
  „Complement triggers relocation of Mortalin/GRP75 frommitochondria to the plasma membrane”, Immunobiology, (2016)

Conference constributions

• H. Grefe, C. Geisler, A. Egner:
  Novel 3D single marker switching microscope with isotropic resolution over large axial range, SPIE Photonics West 2016, Single Molecule Spectroscopy and Superresolution Imaging IX, San Francisco, USA (02.2016)

• A. Egner:
  New developments in optical nanoscopy, International Symposium 2016 "Biological Dynamics from Microscopic to Mesoscopic Scales", Grimma (02.2016)

• R. Siegmund, C. Geisler, A. Egner:
  isoSTED microscopy in living cells, International Symposium 2016 "Biological Dynamics from Microscopic to Mesoscopic Scales", Grimma (02.2016)

• H. Mittelstädt, C. Geisler, A. Egner:
  Single Marker Switching Microscope with Isotropic Resolution over Large Axial Range, Focus on Microscopy 2016, Taipei, Taiwan (03.2016)

• J. Schubert, J. Keller-Findeisen, C. Geisler, B. Vincon, A. Egner:
  Low-intensity STED microscope with increased image brightness and uncompromised resolution, 80. Jahrestagung der DPG und DPG-Frühjahrstagung, Regensburg (03.2016)

• A. Egner:
  Super-resolved fluorescence microscopy: Principle and applications of STED microscopy, BioImaging Symposium 2016, München (04.2016)

• D. Köhne, C. Geisler, P. Simon, A. Egner:
  Principles and applications of optical switching assisted imaging and structuring schemes, International Conference on Physics 2016, New Orleans, USA (06.2016)

• A. Egner, J. Schubert, C. Geisler, B. Vincon:
  Low-intensity STED microscope with increased image brightness and uncompromised resolution, SPIE Nanoscience + Engineering, Nanoimaging and Nanospectroscopy IV, San Diego, USA (08.2016)

• A. Egner:
  Super-resolved fluorescence microscopy: Principle and applications of STED microscopy, 623th Wilhelm and Else Heraeus-Seminar on "Cellular Dynamics", Bad Honnef (09.2016)

• R. Siegmund, F. Rocca, C. Geisler, A. Egner:
  isoSTED microscopy in living cells, International Symposium 2016 "Biological Dynamics from Microscopic to Mesoscopic Scales", Grimma (02.2016)

• A. Egner, A. Munk:
  Statistical reconstruction methods for time varying nanoscale imaging problems, International Symposium 2016 "Biological Dynamics from Microscopic to Mesoscopic Scales", Grimma (02.2016)

• B. Vinçon, J.-R. Krüger, C. Geisler, A. Egner:
  Ultrafast switchable depletion patterns for STED microscopy generated by conical diffraction, 623th Wilhelm and Else Heraeus-Seminar on "Cellular Dynamics", Bad Honnef (09.2016)

• F. Rocca, N. Mazkereth, J.-R. Krüger, C. Geisler, A. Egner, Z. Fishelson:Complement triggers relocation of Mortalin/GRP75 from mitochondria to the plasma membrane, 623th Wilhelm and Else Heraeus-Seminar on "Cellular Dynamics", Bad Honnef (09.2016)

Publications

• A. Hartmann, S. Huckemann, J. Dannemann, O. Laitenberger, C. Geisler, A. Egner, A. Munk:
  Drift Estimation in sparse sequential dynamic imaging: with application to nanoscale fluorescence microscopy, J. R. Stat. Soc. B, doi: 10.1111/rssb.12128 (2015)

• T. Aspelmeier, A. Egner, A. Munk:
  Modern Statistical Challenges in High-Resolution Fluorescence Microscopy, Annu. Rev. Stat. Appl. 2, 163–202 (2015)

Conference contributions

• R. Siegmund, C. Geisler, F. Rocca, C. A. Wurm, S. Jakobs, A. Egner:
  Three Dimensional IsoSTED Microscopy In Living Cells, Focus on Microscopy 2015, Göttingen (03.2015)

• O. Laitenberger, A. Hartmann, C. Geisler, A. Munk, A. Egner:
  Statistical Drift Estimation And Correction Methods For Single Marker Switching Based Microscopy, Focus on Microscopy 2015, Göttingen (03.2015)

• H. Grefe, C. Geisler, A. Egner:
  Novel 3D Single Marker Switching Microscope With Isotropic Resolution Over Large Axial Range, Focus on Microscopy 2015, Göttingen (03.2015)

• A. Egner, C. Geisler, H. Grefe, J. Schubert:
  Developments in fluorescence nanoscopy, Optics + Photonics, Nanoimaging and Nanospectroscopy III, San Diego (08.2015)

• R. Siegmund, C. Geisler, A. Egner:
  isoSTED microscopy in living cells, Optics + Photonics, Nanoimaging and Nanospectroscopy III, San Diego (08.2015)

• A. Egner:
  Super-Resolved Fluorescence Microscopy: Principle and Applications of STED Microscopy, IMB seminar, Tromsø (10.2015)

• A. Egner:
  Angewandte Optik – abbilden, analysieren und strukturieren mit Licht, Bier & Brezeln, Göttingen (11.2015)

• J.-R. Schubert, C. Geisler, A. Egner:
  Low-Intensity STED Microscope With Increased Image Brightness And Uncompromised Resolution, Focus on Microscopy 2015, Göttingen (03.2015)

Publications

• A. Hartmann, S. Huckemann, J. Dannemann, O. Laitenberger, C. Geisler, A. Egner, A. Munk:
  Drift Estimation in sparse sequential dynamic imaging: with application to nanoscale fluorescence microscopy arxiv.org, 1403.1389, (2014)
   
• V. N. Belov, G. Y. Mitronova, M. L. Bossi, V. P. Boyarskiy, E. Hebisch, C. Geisler, K. Kolmakov, C. A. Wurm, K. I. Willig, S. W. Hell:
  Masked Rhodamine Dyes of Five Principal Colors Revealed by Photolysis of a 2-Diazo-1-Indanone Caging Group: Synthesis, Photophysics, and Light Microscopy Applications, Chem. Eur. J. 20, 13162-13173 (2014)

Conference contributions

• A. Egner:
  Overcoming the resolution barrier by STED microscopy Symposium on Advanced Microscopy Techniques in Life Sciences, Homburg (04.2014)

• A. Egner:
  Three‐dimensional optical nanoscopy with opposing lenses SPIE Optics and Photonics, San Diego (08.2014)

• A. Egner:
  Three‐dimensional optical nanoscopy with opposing lenses 18th International Microscopy Congress, Prague (09.2014)

• A. Egner:
  Optical Nanoscopy meets Statistiks: Decoding the Building Blocks of Life Eröffnungssymposium des Felix‐Bernstein Institut für Mathematische Statistik in den Biowissenschaften, Göttingen, (10.2014)

Conference constributions

• A. Egner:
  "Three-dimensional optical nanoscopy with opposing lenses"
  Symposium on „Super Resolution Imaging“, Göttingen (2013)
   
• C. Geisler, R. Schmidt, D. Aquino, S. W. Hell, A. Egner:
  "Developments in Fluorescence Nanoscopy", SPIE Optics + Photonics, San Diego (2013)
   
• C. Geisler, T. Hotz, A. Schönle, S. W. Hell, A. Munk, A. Egner:
  "Drift estimation for single marker switching based imaging schemes"
  SPIE Optics + Photonics, San Diego (2013)
   
• A. Egner:
  "Superresolution Microscopy"
  Fourth Annual Conference "Superresolution Microscopy", Ljubljana (2013)
   
• C. Geisler, J. Jorgačevski, M. Kreft, R. Zorec, A. Egner:
  "Two-color STED microscopy"
  Fourth Annual Conference "Superresolution Microscopy", Ljubljana (2013)
   
• A. Egner:
  "Overcoming the resolution barrier by STED microscopy"
  Visualizing the invisible in cell biology, Symposium on super-resolution microscopy, Prague (2013)

Publications

• C. Geisler, T. Hotz, A. Schönle, S.W. Hell, A. Munk, A. Egner:
  "Drift estimation for single marker switching based imaging schemes"  Opt. Express 20, 7274 (2012)
   
• C. Geisler, T. Hotz, A. Schönle, S.W. Hell, A. Munk, A. Egner:
  "Drift estimation for single marker switching based imaging schemes"
  Opt. Express 20, 7274 (2012)
   
• F. Opazo, M. Levy, M. Byrom, C. Schäfer, C. Geisler, T. W. Groemer, A. D. Ellington, S. O. Rizzoli:
  "Aptamers as potential tools for superresolution microscopy"
  Nat. Meth. 9, 938 (2012)
   
• A. C. Meyer, D. Khimich, A. Egner, T. Moser:
  "Super-resolution optical microscopy of the organ of Corti.
  Investigations on the fine structure of the inner hair cell afferent synapse by the 4Pi and STED techniques."
  HNO 60, 707 (2012)
   
• G. Whelan, E. Kreidl, G. Wutz, A. Egner, J. M. Peters, G. Eichele:
  "Cohesin acetyltransferase Esco2 is a cell viability factor and is required for cohesion in pericentric heterochromatin."
  EMBO J. 31, 71 (2012)

Publications

• D. Aquino, A. Schönle, C. Geisler, C. v. Middendorff, C. A. Wurm, Y. Okamura, T. Lang, S. W. Hell, A. Egner:
  "Two-color nanoscopy of three-dimensional volumes by 4Pi detection of stochastically switched fluorophores"
   Nature Methods, 8 (4), 353 - 359, (2011)
   
• Y. Okamura, R. Schmidt, I. Raschke, M. Hintze, S. Takeoka, A. Egner, T. Lang:
  "A Few Immobilized Thrombins Are Sufficient for Platelet Spreading"
   Biophysical Journal, Volume 100, Issue 8, (2011)
   
• Y. Hua, R. Sinha, C. Thiel, R. Schmidt, J. Huve, H. Martens, S. W. Hell, A. Egner, J. Klingauf:
  "A readily retrievable pool of synaptic vesicles", Nature Neuroscience  doi:10.1038/nn.2838, (2011)
   
• C. A. Wurm, D. Neumann, M. A. Lauterbach, B. Harke, A. Egner, S. W. Hell, S. Jakobs:
  "Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient"
  PNAS, doi:10.1073/pnas.1107553108, (2011)
   
• K. Kumar, L. Isa, A. Egner, R. Schmidt, M. Textor, E. Reimhult:
  "Formation of Nanopore-Spanning Lipid Bilayers through Liposome Fusion!"
  Langmuir, 27, 10920-10928, (2011)
   
• C. K. Ullal, S. Primpke, R. Schmidt, U. Böhm, A. Egner, P. Vana, S. W. Hell:
  "Flexible Microdomain Specific Staining of Block Copolymers for 3D Optical Nanoscopy"
  Macromolecules, 44, 7508-7510, (2011)

Publications

• G. Vicidomini, R. Schmidt, A. Egner, S. W. Hell, A. Schönle:
  "Automatic deconvolution in 4Pi-microscopy with variable phase"
   Opt. Exp. 18 (8), 10154 - 10167, (2010)
   
• C. A. Wurm, D. Neumann, R. Schmidt, A. Egner and S. Jakobs:
  "Sample Preparation for STED Microscopy"
  In: D.B. Papkovsky (ed.), Live Cell Imaging, Methods in Molecular Biology 591, DOI 10.1007/978-1-60761-404-3 11, © Humana Press, a part of Springer Science+Business Media,
  LLC (2010)
   
• T. Frank, M. A. Rutherford, N. Strenzke, A. Neef, T. Pangrsic, D. Khimich, A. Fejtova, E. D. Gundelfinger, M. C. Liberman, B. Harke, K. E. Bryan, A. Lee, A. Egner, D. Riedel, T. Moser:
  "Bassoon and the Synaptic Ribbon Organize Ca2+ Channels and Vesicles to Add Release Sites and Promote Refilling"
  Neuron, 68, 724-738, (2010)

Publications

• S. W. Hell,  R. Schmidt, A. Egner:
  "Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses" (Nature Photonics 3, 381 - 387), (2009)
   
• A. C. Meyer, T. Frank, D. Khimich, G. Hoch, D. Riedel, N. M. Chapochnikov, Y. M. Yarin, B. Harke, S. W. Hell,
  A. Egner, T. Moser:
  "Tuning of synapse number, structure and function in the cochlea", Nature Neuroscience 12 (4), 444-453, (2009)
   
• R. Schmidt, C. A. Wurm, A. Punge, A. Egner, S. Jakobs, S. W. Hell:
  "Mitochondrial Cristae Revealed with Focused Light", Nano Lett. 9 (6), 2508-2510, (2009)
   
• C. K. Ullal, R. Schmidt, S. W. Hell, A. Egner:
  "Block Copolymer Nanostructures Mapped by Far-Field Optics", Nano Lett. 9 (6), 2497-2500 (2009)
   
• G. Perinetti, T. Müller, A. Spaar, R. Polishchuk, A. Luini, A. Egner:
  "Correlation of 4Pi and Electron Microscopy to Study Transport Through Single Golgi Stacks in Living Cells with Super Resolution", Traffic 10, 379-391, (2009)

• R. Schmidt,  C. A. Wurm, S. Jakobs, J. Engelhardt, A. Egner, S. W. Hell:
  "Spherical nanosized focal spot unravels the interior of cells"
  Nature Meth. 5 (6), 539 - 544 (2008)
   
• M. Andresen, A. C. Stiel, J. Fölling, D. Wenzel, A. Schönle, A. Egner, C. Eggeling, S. W. Hell, S. Jakobs:
  "Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy"
  Nature Biotech. 26 (9), 1035-1040 (2008)
   
• A. C. Stiel, M. Andresen, H. Bock, M. Hilbert, J. Schilde, A. Schönle, C. Eggeling, A. Egner, S. W. Hell, S. Jakobs:
  "Generation of Monomeric Reversibly Switchable Red Fluorescent Proteins for Far-Field Fluorescence Nanoscopy"
  Biophys. J. 95, 2989-2997 (2008)
   
• M. Bossi, J. Fölling, V. N. Belov, V. P. Boyarskiy, R. Medda, A. Egner, C. Eggeling, A. Schönle, S. W. Hell:
  "Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species"
  Nano Letters 8 (8), 2463 - 2468 (2008)
   
• C. v. Middendorff, A. Egner, C. Geisler, S. W. Hell, A. Schönle:
  "Isotropic 3D Nanoscopy based on single emitter switching"
   Opt. Expr. 16 (25), 20774-20788 (2008)
   
• I. Testa, A. Schönle, C. v. Middendorff, C. Geisler, R. Medda, C. A. Wurm, A. C. Stiel, S. Jakobs, M. Bossi, C. Eggeling, S. W. Hell, A. Egner:
  "Nanoscale separation of molecular species based on their rotational mobility"
  Opt. Expr. 16 (25), 21093 - 21104 (2008)
   
• J. Fölling, V. Belov, D. Riedel, A. Schönle, A. Egner, C. Eggeling, M. Bossi, S. W. Hell:
  "Fluorescence Nanoscopy with Optical Sectioning by Two-Photon Induced Molecular Switching using Continuous-Wave Lasers"
  ChemPhysChem 9, 321 - 326 (2008)

• A. Egner,  C. Geisler, C. v. Middendorff, H. Bock, D. Wenzel, R. Medda, M. Andresen, A. C. Stiel, S. Jakobs, C. Eggeling, A. Schönle, S. W. Hell:
  "Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters", Biophys. J. 93, 3285 - 3290 (2007)
   
• J. Fölling,  V. Belov, R. Kunetsky, R. Medda, A. Schönle, A. Egner, C. Eggeling, M. Bossi, S. W. Hell:
  "Photochromic Rhodamines provide Nanoscopy with Optical Sectioning"
  Angew. Chem. Int. Ed. 46, 6266 - 6270 (2007)
   
• H. Bock, C. Geisler, C. Wurm, S. Jakobs, A. Schönle, A. Egner, S.W. Hell, C. Eggeling:
  "Two-color far-field fluorescence nanoscopy based on photoswitching emitters", Appl. Phys. B 88, 161-165 (2007)
   
• C. Geisler,  A. Schönle, C. v. Middendorf, H. Bock, C. Eggeling, A. Egner, S.W. Hell:
  "Resolution of Lambda /10 in fluorescence microscopy using fast single molecule photo-switching", Appl. Phys. A 88, 223-226 (2007)
   
• C. Eggeling, M. Hilbert, H. Bock, C. Ringemann, M. Hofmann, A. C. Stiel, M. Andresen, S. Jakobs, A. Egner, A. Schönle, S. W. Hell:
  "Reversible Photoswitching Enables Single-Molecule Fluorescence Fluctuation Spectroscopy at High Molecular Concentration"
  Micr. Res. Techn. 70, 1003 - 1009 (2007)

• A. Egner,  S. W. Hell:
  "Aberrations in Confocal and Multi-Photon Fluorescence Microscopy Induced by Refractive Index Mismatch"
  In: Handbook of Biological Confocal Microscopy, pp. 404-413, Ed. J. Pawley, Springer, New York (2006)
   
• J. Bewersdorf, A. Egner, S. W. Hell:
  "Multifocal Multi-Photon Microscopy"
  In: Handbook of Biological Confocal Microscopy, pp. 550-560, Ed. J. Pawley, Springer, New York (2006)
   
• J. Bewersdorf, A. Egner, S. W. Hell:
  "4Pi Microscopy"
  In: Handbook of Biological Confocal Microscopy, pp. 561-570, Ed. J. Pawley, Springer, New York (2006)

• A. Egner,  S. W. Hell:
  "Fluorescence microscopy with super-resolved optical sections"
  Trends Cell Biol. 15 (4): 207 - 215 (2005)
   
• A. Egner, S. Verrier, A. Goroshkov, H.-D. Söling and S. W. Hell:
  "4Pi-microscopy of the Golgi apparatus in live mammalian cells"
  J. Struct. Biol. 147(1): 70-76 (2004)
   
• S. Jakobs, N. Martini, A. C. Schauss, A. Egner, B. Westermann, S. W. Hell:
  "Spatial and temporal dynamics of budding yeast mitochondria lacking division component Fis1p"
  J. Cell Sci. 116: 2005-2014 (2003)
   
• A. Egner, S. Jakobs and S. W. Hell:
  "Fast 100-nm resolution 3D-microscope reveals structural plasticity of mitochondria in live yeast"
  Proc. Natl. Acad. Sci. USA 99: 3370-3375 (2002)
   
• A. Egner, V. Andresen, S. W. Hell:
  "Comparison of the axial resolution of practical Nipkow-disk confocal fluorescence microscopy with that of multifocal multiphoton microscopy: theory and experiment"
  J. Microsc. 206: 24-32 (2003)
   
• V. Andresen, A. Egner, S. W. Hell:
  "Time-multiplexed multifocal multiphoton microscope"
  Opt. Lett. 26(2): 75-77 (2003)
   
• A. Egner and S. W. Hell:
  "Time multiplexing and parallelization in multifocal multiphoton microscopy"
  J. Opt. Soc. Am. A 17(7): 1192-1201 (2003)
   
• T. Klar, S. Jakobs, M. Dyba, A. Egner, S. W. Hell:
  "Fluorescence microscopy with diffraction resolution limit broken by stimulated emission"
  PNAS 97(15): 8206-8210 (2000)
   
• A. Egner, S. W. Hell:
  "Equivalence of the Huygens-Fresnel and Debye approach for the calculation of high aperture point-spread-functions in the presence of refractive index mismatch"
  J. Microsc. 193(3): 244-249 (1999)
   
• A. Egner, M. Schrader, S. W. Hell:
  "Refractive index mismatch induced intensity and phase variations in fluorescence confocal, multiphoton and 4Pi-microscopy"
  Opt. Commun. 153: 211-217 (1998)